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a549-hace2-tmprss2 cells  (InvivoGen)


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    InvivoGen a549-hace2-tmprss2 cells
    A549 Hace2 Tmprss2 Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 5884 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a549+hace2+tmprss2+cells/A549-hACE2-TMPRSS2+Cells/custom%40a549-hace2tpsa%4010%2E64898%2F2026%2E08%2E14%2E744970
    Average 96 stars, based on 5884 article reviews
    a549-hace2-tmprss2 cells - by Bioz Stars, 2026-09
    96/100 stars

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    In Vitro:

    Article Title: Remdesivir and Obeldesivir Retain Potent Antiviral Activity Against SARS-CoV-2 Omicron Variants.
    Article Snippet: .. To evaluate the in vitro antiviral activity of RDV and ODV against the Omicron variants BA.2.86, BF.7, BQ.1, CH.1.1, EG.1.2, EG.5.1, EG.5.1.4, FL.22, HK.3, HV.1, JN.1, XBB.1.5, XBB.1.5.72, XBB.1.16, XBB.2.3.2, XBC.1.6, and XBF, a previously described SARSCoV-2 anti-nucleoprotein enzyme-linked immunosorbent assay (ELISA) was utilized in A549-hACE2-TMPRSS2 cells (a549-hace2tpsa; InvivoGen, San Diego, CA, USA) [9,38]. ..

    Article Title: Remdesivir and Obeldesivir Retain Potent Antiviral Activity Against SARS-CoV-2 Omicron Variants
    Article Snippet: .. To evaluate the in vitro antiviral activity of RDV and ODV against the Omicron variants BA.2.86, BF.7, BQ.1, CH.1.1, EG.1.2, EG.5.1, EG.5.1.4, FL.22, HK.3, HV.1, JN.1, XBB.1.5, XBB.1.5.72, XBB.1.16, XBB.2.3.2, XBC.1.6, and XBF, a previously described SARS-CoV-2 anti-nucleoprotein enzyme-linked immunosorbent assay (ELISA) was utilized in A549-hACE2-TMPRSS2 cells (a549-hace2tpsa; InvivoGen, San Diego, CA, USA) [ , ]. ..

    Activity Assay:

    Article Title: Remdesivir and Obeldesivir Retain Potent Antiviral Activity Against SARS-CoV-2 Omicron Variants.
    Article Snippet: .. To evaluate the in vitro antiviral activity of RDV and ODV against the Omicron variants BA.2.86, BF.7, BQ.1, CH.1.1, EG.1.2, EG.5.1, EG.5.1.4, FL.22, HK.3, HV.1, JN.1, XBB.1.5, XBB.1.5.72, XBB.1.16, XBB.2.3.2, XBC.1.6, and XBF, a previously described SARSCoV-2 anti-nucleoprotein enzyme-linked immunosorbent assay (ELISA) was utilized in A549-hACE2-TMPRSS2 cells (a549-hace2tpsa; InvivoGen, San Diego, CA, USA) [9,38]. ..

    Article Title: Antiviral Activity of Remdesivir and Obeldesivir Against SARS-CoV-2 Omicron Subvariants That Were Circulating from September 2023 Through June 2025
    Article Snippet: .. To evaluate the antiviral activity (half-maximal effective concentration [EC 50 ]) of RDV and ODV against clinical isolates of Omicron subvariants BA.2.86.1, JN.1.7, KP.2, KP.3.1.1, KP.3.3, XBB.2, and XEC , a nucleoprotein enzyme-linked immunosorbent assay (ELISA) in A549-hACE2-TMPRSS2 cells (a549-hace2tpsa; InvivoGen, San Diego, CA, USA) was used, as described previously [ , , ]. ..

    Article Title: Antiviral Activity of Remdesivir and Obeldesivir Against SARS-CoV-2 Omicron Subvariants That Were Circulating from September 2023 Through June 2025.
    Article Snippet: .. To evaluate the antiviral activity (half-maximal effective concentration [EC50]) of RDV and ODV against clinical isolates of Omicron subvariants BA.2.86.1, JN.1.7, KP.2, KP.3.1.1, KP.3.3, XBB.2, and XEC (Table A1), a nucleoprotein enzyme-linked immunosorbent assay (ELISA) in A549-hACE2-TMPRSS2 cells (a549-hace2tpsa; InvivoGen, San Diego, CA, USA) was used, as described previously [7,10,11]. ..

    Article Title: Remdesivir and Obeldesivir Retain Potent Antiviral Activity Against SARS-CoV-2 Omicron Variants
    Article Snippet: .. To evaluate the in vitro antiviral activity of RDV and ODV against the Omicron variants BA.2.86, BF.7, BQ.1, CH.1.1, EG.1.2, EG.5.1, EG.5.1.4, FL.22, HK.3, HV.1, JN.1, XBB.1.5, XBB.1.5.72, XBB.1.16, XBB.2.3.2, XBC.1.6, and XBF, a previously described SARS-CoV-2 anti-nucleoprotein enzyme-linked immunosorbent assay (ELISA) was utilized in A549-hACE2-TMPRSS2 cells (a549-hace2tpsa; InvivoGen, San Diego, CA, USA) [ , ]. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Remdesivir and Obeldesivir Retain Potent Antiviral Activity Against SARS-CoV-2 Omicron Variants.
    Article Snippet: .. To evaluate the in vitro antiviral activity of RDV and ODV against the Omicron variants BA.2.86, BF.7, BQ.1, CH.1.1, EG.1.2, EG.5.1, EG.5.1.4, FL.22, HK.3, HV.1, JN.1, XBB.1.5, XBB.1.5.72, XBB.1.16, XBB.2.3.2, XBC.1.6, and XBF, a previously described SARSCoV-2 anti-nucleoprotein enzyme-linked immunosorbent assay (ELISA) was utilized in A549-hACE2-TMPRSS2 cells (a549-hace2tpsa; InvivoGen, San Diego, CA, USA) [9,38]. ..

    Article Title: Antiviral Activity of Remdesivir and Obeldesivir Against SARS-CoV-2 Omicron Subvariants That Were Circulating from September 2023 Through June 2025
    Article Snippet: .. To evaluate the antiviral activity (half-maximal effective concentration [EC 50 ]) of RDV and ODV against clinical isolates of Omicron subvariants BA.2.86.1, JN.1.7, KP.2, KP.3.1.1, KP.3.3, XBB.2, and XEC , a nucleoprotein enzyme-linked immunosorbent assay (ELISA) in A549-hACE2-TMPRSS2 cells (a549-hace2tpsa; InvivoGen, San Diego, CA, USA) was used, as described previously [ , , ]. ..

    Article Title: Antiviral Activity of Remdesivir and Obeldesivir Against SARS-CoV-2 Omicron Subvariants That Were Circulating from September 2023 Through June 2025.
    Article Snippet: .. To evaluate the antiviral activity (half-maximal effective concentration [EC50]) of RDV and ODV against clinical isolates of Omicron subvariants BA.2.86.1, JN.1.7, KP.2, KP.3.1.1, KP.3.3, XBB.2, and XEC (Table A1), a nucleoprotein enzyme-linked immunosorbent assay (ELISA) in A549-hACE2-TMPRSS2 cells (a549-hace2tpsa; InvivoGen, San Diego, CA, USA) was used, as described previously [7,10,11]. ..

    Article Title: Remdesivir and Obeldesivir Retain Potent Antiviral Activity Against SARS-CoV-2 Omicron Variants
    Article Snippet: .. To evaluate the in vitro antiviral activity of RDV and ODV against the Omicron variants BA.2.86, BF.7, BQ.1, CH.1.1, EG.1.2, EG.5.1, EG.5.1.4, FL.22, HK.3, HV.1, JN.1, XBB.1.5, XBB.1.5.72, XBB.1.16, XBB.2.3.2, XBC.1.6, and XBF, a previously described SARS-CoV-2 anti-nucleoprotein enzyme-linked immunosorbent assay (ELISA) was utilized in A549-hACE2-TMPRSS2 cells (a549-hace2tpsa; InvivoGen, San Diego, CA, USA) [ , ]. ..

    Concentration Assay:

    Article Title: Antiviral Activity of Remdesivir and Obeldesivir Against SARS-CoV-2 Omicron Subvariants That Were Circulating from September 2023 Through June 2025
    Article Snippet: .. To evaluate the antiviral activity (half-maximal effective concentration [EC 50 ]) of RDV and ODV against clinical isolates of Omicron subvariants BA.2.86.1, JN.1.7, KP.2, KP.3.1.1, KP.3.3, XBB.2, and XEC , a nucleoprotein enzyme-linked immunosorbent assay (ELISA) in A549-hACE2-TMPRSS2 cells (a549-hace2tpsa; InvivoGen, San Diego, CA, USA) was used, as described previously [ , , ]. ..

    Article Title: Antiviral Activity of Remdesivir and Obeldesivir Against SARS-CoV-2 Omicron Subvariants That Were Circulating from September 2023 Through June 2025.
    Article Snippet: .. To evaluate the antiviral activity (half-maximal effective concentration [EC50]) of RDV and ODV against clinical isolates of Omicron subvariants BA.2.86.1, JN.1.7, KP.2, KP.3.1.1, KP.3.3, XBB.2, and XEC (Table A1), a nucleoprotein enzyme-linked immunosorbent assay (ELISA) in A549-hACE2-TMPRSS2 cells (a549-hace2tpsa; InvivoGen, San Diego, CA, USA) was used, as described previously [7,10,11]. ..



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    Image Search Results


    (A-C) Neutralization of infectious SARS-CoV-2 following incubation with two-fold dilutions of rCL-11 (black) in A549-hACE-2 cells (starting at 40 µg/ml) (A) , Calu-3 cells (starting at 20 µg/ml) (B) , and Huh7.5 cells (starting at 20 µg/ml) (C) using the conventional experimental setup. The mAb clone 61 was used as a neutralization control (orange) and rCL-11 buffer (gray) in corresponding dilutions was used to monitor buffer-mediated effects on the cells. The percentage of protected cells was determined from the number of SARS-CoV-2 infected cells in experimental wells compared to the number of infected cells in virus-only control wells after anti-S protein immunostaining. Data are shown as means ± SD of four (A549-hACE-2 and Calu-3 cells) or six (Huh7.5 cells) replicates.

    Journal: PLOS Pathogens

    Article Title: Collectin-11, a complement pattern recognition molecule, mediates pulmonary SARS-CoV-2 neutralization and protection

    doi: 10.1371/journal.ppat.1014216

    Figure Lengend Snippet: (A-C) Neutralization of infectious SARS-CoV-2 following incubation with two-fold dilutions of rCL-11 (black) in A549-hACE-2 cells (starting at 40 µg/ml) (A) , Calu-3 cells (starting at 20 µg/ml) (B) , and Huh7.5 cells (starting at 20 µg/ml) (C) using the conventional experimental setup. The mAb clone 61 was used as a neutralization control (orange) and rCL-11 buffer (gray) in corresponding dilutions was used to monitor buffer-mediated effects on the cells. The percentage of protected cells was determined from the number of SARS-CoV-2 infected cells in experimental wells compared to the number of infected cells in virus-only control wells after anti-S protein immunostaining. Data are shown as means ± SD of four (A549-hACE-2 and Calu-3 cells) or six (Huh7.5 cells) replicates.

    Article Snippet: The human lung adenocarcinoma A549-hACE-2 cell line (InvivoGen, San Diego, California, USA) (A549 cells expressing human ACE-2) was maintained as described previously [ ].

    Techniques: Neutralization, Incubation, Control, Infection, Virus, Immunostaining